Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific TE Buffer, 1X, 1 L
TE Buffer, 1X, is a ready-to-use buffered solution formulated for a wide range of molecular biology applications. This 1L bottle is ideal for preparing buffers for routine experiments, including nucleic acid extraction, PCR, and gel electrophoresis. The TE Buffer maintains a stable pH and osmolarity, which is essential for protecting nucleic acids and ensuring reliable results during processing. The 1X concentration is optimal for immediate use in applications that require a stable buffering system to maintain the integrity of RNA, DNA, and other biochemical compounds. It is commonly used in laboratories for standard molecular protocols and can be stored for extended periods without degradation.
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661046.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are solid tablets that dissolve easily to prepare 100 mL of phosphate-buffered saline. These tablets provide a convenient and efficient way to prepare PBS solutions, eliminating the need for weighing and measuring individual components. They are ideal for laboratory applications that require consistent and reliable buffer preparation, including cell culture, microbiological testing, and molecular biology procedures. The tablets dissolve quickly in water, ensuring that the resulting buffer maintains the correct pH and osmolarity for a wide range of applications. Each bottle contains 100 tablets, making it a cost-effective option for labs that require frequent PBS preparation.
![Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502387606.png-250.jpg)
Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.
1.54M Sodium Chloride,55mM Sodium Phosphate Dibasic,12mM Potassium Phosphate Monobasic
Alkali Scientific CellPro™ PBS Buffer, 1X pH 7.2, 100mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
![Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/default.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 100 mL Tablets, 500 Tablets per Bottle
PBS [Phosphate Buffered Saline] 100 mL Tablets are designed to make buffer preparation simple and efficient. Each tablet dissolves easily in water to create 100 mL of phosphate-buffered saline. With 500 tablets per bottle, these tablets are perfect for laboratories with high-volume buffer preparation needs. The ease of use and consistency of the PBS solution make these tablets ideal for various applications, including cell culture, microbiological analysis, and molecular biology experiments. The tablets provide a reliable way to ensure that each PBS solution is accurately prepared with the correct pH and osmolarity, minimizing the potential for human error in buffer formulation.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
![Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661850.jpg-250.jpg)
Alkali Scientific DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
DPBS 1X (Dulbecco's Phosphate Buffered Saline) is a sterile saline solution used in biological research and clinical laboratories. The 500mL bottle is a convenient size for smaller laboratories or specific experiments that require moderate volumes of buffer solution. DPBS is widely used for washing cells, preparing tissues, and diluting reagents in cell culture, molecular biology, and microbiology applications. The balanced composition of salts and phosphate ensures that the solution maintains osmotic pressure and pH stability, making it essential for maintaining cell viability and supporting biological assays that require precise environmental conditions.
Biotium Fixation Permeabilization Other Buffers Buffers Accessories and Background Reducers Fixation Permeabilization and Other Buffers Chemical Reagents 50/PK
PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer Box includes 50 packets
![Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661556.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 980g make, 10 L
PBS [Phosphate Buffered Saline] 10X Powder Concentrate, in a 980g pack, makes 10 liters of 10X PBS solution. This highly concentrated powder is perfect for laboratories that require large volumes of PBS for a wide range of biological and biochemical applications. The powder dissolves easily in water, providing a quick and convenient way to prepare the solution. 10X PBS is essential in many laboratory protocols, including protein assays, cell culture, and washing procedures. The larger pack size is ideal for high-throughput laboratories or research facilities that need to prepare PBS solutions in bulk for routine use in experiments and testing.
ILEX LIFE SCIENCES LLC GELATIN SOLUTION 2.0 100 ML
PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg (100 ml). DESCRIPTION: PAN-Biotech Gelatin Solution 2% in DPBS, w/o: Ca, Mg for laboratory research applications. Gelatin is a heterogenous mixture of water soluble proteins of high average molecular weight present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, or bones in water. APPLICATION: Applications using gelatin include coating cell culture plates to improve cell attachment for a variety of cell types, addition to PCR to stabilize Taq DNA Polymerase, and use as a blocking agent in Western Blotting, ELISA, and immunohistochemistry. In bacteriology, gelatin can be used as a component of culture media. STORAGE: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Apexbio Technology LLC Annexin V-HF488/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes Propidium iodide (PI) selectively binds double-stranded DNA and yields a red fluorescence PI is membrane-impermeable in healthy cells but can penetrate the membranes of late apoptotic and dead cells staining their nuclei Simultaneous staining with Annexin V-HF488 and PI enables differentiation between early apoptotic late apoptotic and dead cells
Biotang Inc Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S
Sterile PBS, 500 ml: 10 mM Sodium phosphate dibasic, 2 mM potassium phosphate monobasic, 2.7 mM potassium chloride, 137 mM sodium chloride, pH 7.4 +/- 0.2. Prepared in 18.2 megohms water. 0.22-micron filtered and/or autoclaved. BM-220S.
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on