Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 25 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is ideal for laboratory applications requiring precise pH regulation. ADA is commonly used in chromatography, protein studies, and enzyme assays, providing a stable pH environment during various biochemical experiments. Its ability to maintain a controlled pH is essential for accurate results in protein purification, cell culture, and molecular biology studies. The 25g size is perfect for smaller-scale experiments or routine laboratory use where reliable buffering capacity is necessary for optimal results.
Quality Biological Inc TE pH 8.0, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immunohistochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
![Alkali Scientific TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660713.jpg-250.jpg)
Alkali Scientific TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
TAE 50X Buffer (TRIS Acetate EDTA) is an ultra-concentrated solution used to prepare working buffers for high-resolution nucleic acid electrophoresis. The 1-liter bottle provides a 50X concentration that can be diluted to the required 1X concentration by adding the appropriate amount of water. This buffer is widely used in molecular biology for techniques such as agarose gel electrophoresis, where its low ionic strength minimizes the diffusion of nucleic acid fragments, allowing for better separation. The 50X concentration ensures that laboratories can prepare large quantities of TAE buffer for routine experiments while maintaining buffer performance and stability.
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 10L Sto
Alkali Scientific MOPSO Sodium Salt, 1 Kg
MOPSO Sodium Salt, 1 Kg, is an essential buffering agent used to maintain pH stability in a variety of laboratory applications, especially in protein and nucleic acid studies. The MOPSO buffer provides reliable pH control in the range of 6.5 to 7.9, making it an ideal choice for enzyme assays, protein extractions, and other biochemical processes. The 1 kg size is perfect for large-scale experiments or labs that require frequent use of this buffer for ongoing research. MOPSO is also widely used in pharmaceutical and biotechnology labs, where consistent pH levels are crucial for reproducible results in complex biological assays.
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 20ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (20 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Alkali Scientific CellPro™ PBS 1X, pH 7.4, withSodium Azide, FAcs Washing Grade, 20L Cubitainer
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Research Products International Corp TRIS-BORATE-EDTA, 10X SOLUTION, 1 LITER
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Quality Biological Inc TE pH 7.4, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt 99 titrati25G
MES sodium salt is commonly employed as buffer. Useful pH range for MES sodium salt is 5.5 to 6.7.
Teknova 20mM HEPES, 150mM Sodium Chloride, pH 7.0. 1000mL. Endotoxin Tested, Sterile. 8 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1X PBS with 200mM Sodium Chlor
1X PBS with 200mM Sodium Chloride, 0.001% P-188, 1000mL, Sterile 8 pack.
Quality Biological Inc TBE Buffer 10X, 1000 mL
TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. ¿ Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. ¿ Applications: TBE Buffer, 1X is used for DNA electrophoresis. ¿ TBE Buffer, 0.5X is used for electrophoresis onto DEAE cellulose membrane. ¿ TBE Buffer, 2X is used for polyacrylamide and agarose electrophoresis. ¿ TBE is also used for other nucleic acid electrophoresis procedures such as Northern and Southern blots. ¿ Storage Conditions: 15 to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months