Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 100g
Zymo Research Corporation ZymoPURE™ Binding Buffer (150 ml)
ZymoPURE™ Binding Buffer (150 ml). Assists in binding nucleic acid to ZymoPURE™ columns for purification.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 5 x 10 pkg
Phosphate buffered saline | MFCD00131855 | 5 x 10 pkg
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, MFCD00132476, 10pk
pH 8.0, powder, Synonym: TBS. Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); pH 8.0, at 25 °C.
Medchemexpress LLC Mops | 1132-61-2 | 99.95% | 25 G
MOPS is a buffering agent used in biology. It is known for its ability to maintain the pH of mammalian cell culture media and significantly improve the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH of mammalian cell culture media.
- Improves thermal stability of BSA.
Sigma Aldrich Fine Chemicals Biosciences Trizma hydrochloride, 1185-53-1, MFCD00012590, 500 g
Linear Formula: NH2C(CH2OH)3 · HCl, Molecular Weight: 157.60, BioPerformance Certified, cell culture tested, ≥99.0% (titration), Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride.
FUJIFILM BIOSCIENCES INC PBS 1X-Dulbecco's Phosphate Buffered Saline Solution - Liquid
PBS 1X Dulbecco's Phosphate Buffered Saline Solution can be used to provide a buffer system to maintain cell culture media in the physiological pH range, or to irrigate, transport, or dilute fluid while maintaining cell tonicity and viability.
Teknova 1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
ProteinSimple Sample Buffer, 440 µL, Compatible with Jess™, Abby™, Wes™, Peggy Sue and Sally Sue systems.
Wes, Sally Sue, Peggy Sue 10X Sample Buffer (440 uL).
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L